Policy & Regulation
City of Hope COVID-19 Investigational Vaccine Produces Strong Immunity in Preclinical Research
1 December 2020 - - A city of Hope investigational vaccine produced strong protective immunity against SARS-CoV-2, including neutralizing antibodies that recognize the current dominant circulating form, which is a mutant of the original Wuhan strain, according to research published in Nature Communications.

City of Hope is a US-based independent biomedical research and treatment center for cancer, diabetes and other life-threatening diseases.

The vaccine is now being tested in a phase 1 clinical trial at city of Hope, an independent biomedical research and treatment center for cancer, diabetes and other life-threatening diseases.

The investigational vaccine generated abundant neutralizing antibodies, which prevented the virus from infecting healthy cells, and produced strong responses by T cells against the virus' spike (S) and nucleocapsid (N) antigens.

The strong T cell response could provide long-term protection that may protect recipients of the vaccine against future SARS-CoV-2 outbreaks.

Diamond and his team also studied whether the mice that received the vaccine would still have immunity if SARS-CoV-2 mutated.

To do that, they inserted various genetic sequences from SARS-CoV-2 into test viruses called pseudoviruses.

The team found that antibodies, generated by the mice in response to the vaccine, prevented infection of susceptible cells by the pseudoviruses.

To produce the investigational vaccine, a team of city of Hope scientists, led by Felix Wussow, Ph.D., and Flavia Chiuppesi, Ph.D., both city of Hope assistant research professors in the Department of Hematology and Hematopoietic Cell Transplantation, together with Diamond, first developed a unique synthetic modified vaccinia ankara platform technology to house the genetic components that make up the vaccine candidate at a molecular level.

MVA is a safely modified poxvirus that is approved by the Food and Drug Administration for the prevention of smallpox and monkeypox, and is also included in the federal government's Strategic National Stockpile for lifesaving use during a public health emergency.

For the COVID-19 investigational vaccine, the platform was used to rapidly produce the synthetic virus, which then started to replicate its DNA within a cell.

The virus produced large quantities of the SARS-CoV-2 S protein, which led to the production of neutralizing antibodies against the virus, and the N antigen.

Both the S and N antigens also elicited a strong T cell response for the mice to mount an immune response against the virus. All mice who received the vaccine produced these results with no side effects.

The MVA platform has similarities to the Triplex vaccine developed by city of Hope against cytomegalovirus, a herpesvirus that can cause complications for stem cell transplant recipients.

Research published in Annals of Internal Medicine showed that patients in the phase 2 randomized multicenter trial who received Triplex were less likely to develop health complications related to the herpesvirus than patients who did not take it.

The p53MVA vaccine, also developed at city of Hope, is continuing to be used in combination with cancer drugs, such as pembrolizumab and gemcitabine, against solid tumors.

Previous experience with MVA-based vaccines has shown few side effects due to the highly attenuated virus-based vaccine makeup.

city of Hope aims to complete its phase 1 clinical trial by the end of March 2021.

city of Hope is an independent biomedical research and treatment center for cancer, diabetes and other life-threatening diseases.

Founded in 1913, city of Hope is in bone marrow transplantation and immunotherapy such as CAR T cell therapy.

city of Hope's translational research and personalized treatment protocols advance care throughout the world. Human synthetic insulin and numerous breakthrough cancer drugs are based on technology developed at the institution.

A National Cancer Institute-designated comprehensive cancer center and a founding member of the National Comprehensive Cancer Network, city of Hope has been ranked among the nation's "Best Hospitals" in cancer by US News and World Report for 14 consecutive years.

Its main campus is located near Los Angeles, with additional locations throughout Southern California.
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